Aparicio Lab

Academic

Job Category

Faculty Non Bargaining

Job Title

Postdoctoral Research Fellow - Single Cell Genomics, drug resistance in cancer

Department

Aparicio Laboratory | Department of Pathology and Laboratory Medicine | Faculty of Medicine (Samuel Aparicio)

JOB DESCRIPTION:

Post-doctoral Fellow (PDF) Single Cell Genomics, drug resistance in cancer We are seeking a highly-motivated post-doctoral fellow (PDF) with strong leadership ability and expertise in single cell genomics and transcriptomics, and a strong background in molecular biology. The successful applicant will work full-time under the supervision of Dr. Samuel Aparicio on an exciting project decoding the role of epigenomic and genomic drug resistance. The position start date is flexible.

JOB SUMMARY:
The Aparicio lab is developing new single cell genome and transcriptome methods for tracking clonal trajectories at single cell level in patient tissues and patient-derived xenografts (PDX). The lab is highly multidisciplinary and highly productive (Laks et al, Cell 2019, Salehi et al Nature 2021, Funnell et al Nature 2022) and is well funded for single cell analysis of genomes and transcriptomes in cancer. The Aparicio lab is developing new single cell genome and transcriptome methods for tracking clonal trajectories at single cell level in patients and patient-derived xenografts. The project involves collaborating on an exciting project mapping clonal dynamics in human cancers under drug treatment with new single cell methods. The post holder will also have the opportunity to interact as part of the international Welcome LEAP Delta Tissue project and an exciting new program in the biology of epithelium from high risk carriers of BRCA1/BRCA2 mutations. These projects are bringing new measurement methods and computational techniques to the 3D reconstruction of tumours, and mapping drug sensitivity and drug combinations using sgRNA/CRISPR methods in vivo. The program works closely with UBC departments of statistics and computer science and the New York Genome Center NY, on the development of new models for single cell data and cancer evolution. Some experience with bioinformatics approaches to biological data and familiarity with handling of genomics data will be an advantage.

ORGANIZATIONAL STATUS:

Aparicio Lab is located in the department of Molecular Oncology at BC Cancer Research Center in Vancouver, British Columbia, Canada. The candidate will report directly to Dr. Samuel Aparicio, and will play a senior leadership role in the laboratory.

The Department of Pathology and Laboratory Medicine at UBC is a hybrid, academically intensive Department within the UBC Faculty of Medicine whose activities span a broad spectrum of teaching, research, and academic service, often performed in the milieu of clinical practice, and are ultimately devoted to improving the care, treatment, and well-being of patients.  The Department offers academic degrees at the bachelor’s (Bachelor of Medical Laboratory Science (BMLSc)) and graduate (MSc, PhD) levels with the graduate program, one of the largest in the Faculty of Medicine, which has been recognized by UBC for its quality. The Department plays a major role in the MD undergraduate program and offers an accredited residency-training program. Faculty members participate across a spectrum of research from basic investigative to translational to clinical applied research and are recognized locally, nationally and internationally for their excellence. 

WORK PERFORMED:
● Provide leadership, strategic direction, and translational research pertaining to single cell genomics and transcriptomics, and CRISPR methods.
● Design and implement research plan to investigate the clonal dynamics in human cancer through single cell methods.
● Design and conduct animal experiments.
● Conduct in vivo testing or characterization of drugs, small molecules, knockouts or antibodies in relevant PDX animal models.
● Foster research collaborations and work jointly with academic, clinical, industry, and other partners.
● Scientifically document lab experiments, data analysis, interpretations.
● Write manuscripts, present results at internal and external meetings.
● Identify funding opportunities and if required write or assist with grant proposals
● Lead and mentor junior trainees/students in the laboratory

CONSEQUENCE OF ERROR:
This is a senior research position that plays a critical role in the development of this novel field. PDF is accountable for the quality and integrity of the research and data, including analyses and interpretation, as well as the effective and efficient management of research projects and deliverables. This position requires innovation, strategic planning, and adaptability. Incorrect decisions or actions may damage the reputation of the laboratory, lead to loss of credibility in this field, and be financially costly.

SUPERVISION RECEIVED:

The PDF will work with a high degree of independence and set priorities under broad directives from Dr. Aparicio. The PDF will be expected to provide direction and mentoring in the laboratory under the supervision of Dr. Aparicio. This position will also provide the candidate with a high level of exposure to single cell genomics and translational research, as well as, providing opportunities to work collaboratively with industry partners and other researchers globally.

SUPERVISION GIVEN:

The PDF will be expected to provide mentorship and guidance to undergraduate, graduate students and other research members as required.

QUALIFICATION:
● Ph.D. in biology, cell biology, molecular biology, biochemistry, genetics, genomics, or similar discipline.
● The candidate should have a solid scientific and technical background with excellent written/oral communication, interpersonal and organizational skills.
● Preference will be given to candidates with a proven track record with a minimum of 2-3 first author publications in reputable journals.
● Proficiency in cell biology, molecular biology, histology, genetics, genomics, next-generation sequencing and biochemistry techniques.
● Demonstrate the ability to work in a highly interdisciplinary environment.
● Experience with cancer research.
● Expertise and hands-on experience in animal studies, including all aspects of design, handling, breeding, tissue collection, histology, and analyses are essential.
● Familiarity with testing/characterization of small molecule or drug in vivo is preferred.
● Previous biochemical/cell biology and molecular biology experience with single cell genomics / transcriptomics, next generation sequencing, drugs screen and PDX modeling is an asset.
● Experience with statistical analyses and scientific writing. Previous experience with grant writing is an asset.
● Experience with bioinformatics approaches to biological data and familiarity with handling of genomics data is an asset.
● Applicants with experience and a strong track record with large drug target screening, single cell methods, and PDX mouse models are especially encouraged to apply.

How to Apply:

Exceptional candidates should submit a cover letter outlining your research interests and fit with the laboratory's research, curriculum vitae, and the names of three referees willing to provide letters of reference.

Due to the number of resumes we receive, we are unable to confirm receipt of submissions over the phone, or provide the status of the competition. Only shortlisted candidates will be contacted.

This position is located within a health-care facility. Therefore, this positions requires successful verification of full vaccination against Covid-19 provided prior to the start date, as required by the provincial health mandate.

UBC hires on the basis of merit and is committed to employment equity. All qualified persons are encouraged to apply.

Equity and diversity are essential to academic excellence.  An open and diverse community fosters the inclusion of voices that have been underrepresented or discouraged.  We encourage applications from members of groups that have been marginalized on any grounds enumerated under the B.C. Human Rights Code, including sex, sexual orientation, gender identity or expression, racialization, disability, political belief, religion, marital or family status, age, and/or status as a First Nation, Metis, Inuit, and/or Indigenous person.

Job Summary
Researchers in the Department of Molecular Oncology at BC Cancer Research Institute use the latest technologies to rapidly identify genes that are involved in the development of cancer. We are seeking a highly-motivated, organized, driven individual with an interest or past experience with working with research animals in an academic setting. This entry level position will provide the successful candidate with an opportunity to work and learn within an academic research environment where continual training and learning is greatly encouraged. The core focus of this position will be handling and working with research animals, as well as, conducting research experiments on animals; hands-on training will be provided to the successful candidate. This animal tech position will be involved with performing research in drug pharmacology directed at human tumours, with assays in immunodeficient mice (xenotransplants).

Organizational Status
The successful applicant will report to the Principal Investigator or his/her delegate and may assist in overseeing technical work of students and trainees.

Work Performed

• Perform and/or oversee animal procedures involving immunization, oral gavage, injections, tumour inoculation, tumour volume measurements, blood and tissue collection, surgical procedures, necropsy, and post-operative care.
• Assist in the daily care of transgenic animals and maintains colonies of human tumour bearing immunodeficient animals.
• Daily health monitoring and care of laboratory animals.
• Plan and perform animal experiments utilizing complex procedures and/or techniques.
• Be able to recognize and resolve behavioral and/or housing issues; may provide technical instructions and training for other staff and/or trainees
• Process tissue samples and carry out primary cell isolation as instructed.
• Carry out laboratory techniques following standard operating protocols ages, tables, graphs and charts.
• Maintain both laboratory and laboratory records of both animal data and experimental data; when required to summarize results in research reports and lab presentations.
• Perform other related duties as assigned.
• On a rotation basis, must be available for emergency calls 24/7 and respond appropriately.

Minimum Qualifications
Completion of a relevant technical program or a university degree in a relevant discipline and a minimum three years of related experience or an equivalent combination of education and experience.

• Willingness to respect diverse perspectives, including perspectives in conflict with one’s own
• Demonstrates a commitment to enhancing one’s own awareness, knowledge, and skills related to equity, diversity, and inclusion

Preferred Qualifications

• Postgraduate degree in Science is preferred.
• CALAS certification (e.g. RLAT) is an asset.
• Minimum of 3 years related experience or the equivalent combination of education and experience.
• Experience with cell culture and aseptic technique preferred, involving both isolation and maintenance of rodent primary cultures and cell lines.
• Experience in the use and handling of hazardous materials such as biological agents and cytotoxic drugs is essential.
• Experience in the monitoring and management of human tumour bearing animals.
• A working knowledge of the CCAC guidelines and compassion and sensitivity for animals.
• Strong interpersonal skills and the ability to interact positively and productively with other team members are essential.
• Ability to communicate effectively both orally and in writing.
• The successful candidate must be well-organized, conscientious, understand the importance of detail, and be able to multi-task and prioritize duties effectively.

JOB SUMMARY

Researchers in the Department of Molecular Oncology at the BC Cancer Research Centre use the latest technologies to rapidly identify genes that are involved in the development of cancer. You will become an integral part of the animal research team, providing technical support on animal research for multiple core projects within the lab.

DUTIES/ACCOUNTABILITIES

• Perform a variety of established technical procedures according to written protocols.
• Perform experiments by following established laboratory procedures according to written protocols.
• Record results, including computer data entry.
• Provide input regarding modification of procedures as requested by the Department Head, Principal Investigator, or designate.
• Maintain and store cell lines and primary cultures.
• Maintain and store test materials and products.
• Maintain inventory of laboratory supplies and equipment.
• May demonstrate work methods or procedures to less experienced assistants as designated.

QUALIFICATIONS

• A level of education, training, and experience equivalent to graduation from a recognized Bachelor’s program in an appropriate discipline (e.g. Bachelor of Science)
• Two (2) years of recent related experience in a research laboratory
• As this role will be spending the majority of the time in an animal research facility working with mice – mice handling experience is preferred.