This position involves with providing technical support in the areas of molecular biology, biochemistry and cell biology for researchers on current on-going research projects. Individual should possess a strong molecular biology and cell biology background, with experience in tissue culture, microscopy, use of confocal and fluorescence microscopes. Recent hands-on experience with antibody labeling and immunohistochemistry will be considered an asset.
The candidate will get exposure to breast and childhood cancers through working directly with two (Dr. Aparicio/Dr. Sorensen) different labs within the department. The Aparicio lab uses the latest technologies to rapidly identify genes that are involved in the development of breast cancer. Through the use of single cell genome and transcriptome methods for tracking clonal trajectories at single cell level in patient tissues, we hope to gain a better understanding of tumor microenvironment.
The Sorensen lab uses a combination of genetic and biochemical approaches to identify proteins that are specifically altered in human tumors. This led to the discovery of many novel genetic alterations in childhood cancer and breast tumors. These discoveries have been translated into new diagnostic tests for specific tumors, and have advanced our understanding of how the involved proteins transmit signals that cause cells to become cancerous. Dr Sorensen is part of two international teams, known as the Pediatric Immunotherapy Discovery and Development Network and the St. Baldrick’s Foundation-SU2C Pediatric Cancer Dream Team, focused on identifying and developing new immunotherapies for high-risk childhood cancers.
The successful applicant will work full-time under the direction, guidance and supervision of both Dr. Samuel Aparicio and Dr. Poul Sorensen, Principal Investigators. The position will provide the candidate with exposure to breast and childhood cancers, and translational research, advanced cutting-edge microscopy technology and techniques, as well as, providing opportunities to work collaboratively with industry partners and other researchers globally; position start date is flexible.
Aparicio and Sorensen Labs are located in the department of Molecular Oncology at BC Cancer Research Institute in Vancouver, British Columbia, Canada. The candidate will report directly to Dr. Samuel Aparicio, Dr. Poul Sorensen or Laboratory and Research Operation Manager, in addition, the applicant may assist in overseeing technical work of students and trainees.
- Plans and performs experiments utilizing established complex standard operating procedures (SOP) and/or techniques for investigating breast cancer.
- Maintain cell cultures and generation of new cell line using aseptic techniques.
- Track samples, record data and work a LMIS systems.
- Provide technical expertise and support in molecular biology, cell biology, protein biochemistry, microscopy and tissue culture; including but not limited to techniques such as PCR, western blotting, cell culture, use of confocal and fluorescent microscope, antibody labeling and immunohistochemistry.
- Maintain and operation large shared infrastructure equipment.
- Assist in developing and implementing standard operating protocols (SOPs).
- Maintains laboratory records and experimental data; when require summarize results in research reports and lab presentations.
- Assist in general maintenance, reagent preparation, receiving and inventory of lab supplies.
- Carries out any other related duties as assigned or required in keeping with the qualifications and requirements of this level of technician.
CONSEQUENCE OF ERROR
The work is of a complex nature and required high level of training, tasks are assigned and the incumbent exercises a considerable amount of responsibility, initiative and judgment in determining own work schedule to achieve those tasks. Errors may result in the permanent loss of irreplaceable tissue samples, loss of thousands of dollars of material and/or damage to equipment. Incorrect decisions or actions may damage the reputation of the laboratory, lead to loss of credibility in this field, and be financially costly. Noncompliance with biosafety and chemical safety regulations may also result in closure of facility or loss of grant funding.
The successful applicant will work under general/minimum supervision. From time to time, Principal Investigator(s), direct supervisor(s) or Laboratory Research and Operations Manager may provide specific instructions on unusual problems, or new project outlines. This position will report to the Principal Investigator(s) or Laboratory Research and Operations Manager or designate.
Depending on experience and expertise, the successful applicant maybe be required to assist in training and supervision of graduate students/trainees, including providing instruction on use of lab equipment and procedures, and will help troubleshoot experiments in their area of expertise.
- A MSc in Biological Sciences (Molecular Biology or other relevant field) or BSc with 1-2 years of related experience in academic research or biotech industry settings, preference will be given to applicants with work experience in microscopy, antibody labeling and cell biology.
- A working knowledge and understanding of microscopy, cell biology and biochemistry techniques such as confocal and fluorescent microscope, cell culture, immunohistochemistry, antibody labeling, PCR, protein purification and western blotting techniques are essential; prior hands-on working experience in antibody labeling and immunohistochemistry is considered a great asset.
- Previous hands-on experience with Fluidigm Hyperion Imaging mass cytometry or Fluidgim CyToF2 or equivalent imaging equipment would be highly valued and considered great asset.
- Hands-on experience with cell culture and aseptic technique is preferred.
- Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques including the following: RNA/DNA/protein isolation, flow cytometry, immunohistochemistry, ELISA, antibody labeling, and western blotting. Experience in immunocytochemistry and antibody labelling would be an asset.
- Demonstrated understanding of experimental design and assay optimization.
- Strong interpersonal skills, communication skills (orally and written) and the ability to interact positively and productively with other team members are essential.
- Excellent documentation and organizational skills.
- The successful candidate must be well-organized, conscientious, understand the importance of detail, and be able to multi-task and prioritize duties effectively.
This is a one year appointment and depending on funding and successful progress of the research it may be renewed for additional years.
UBC hires on the basis of merit and is committed to employment equity. All qualified persons are encouraged to apply. UBC is strongly committed to diversity within its community and especially welcomes applications from visible minority group members, women, Aboriginal persons, persons with disabilities, persons of any sexual orientation or gender identity, and others who may contribute to the further diversification of ideas. However, Canadians and permanent residents of Canada will be given priority.
We thank all interested applicants for their interest, however, only those applicants short-listed will be contacted for an interview.