The Molecular and Cellular Immunology Core (MCIC) is a full service facility specializing in advanced molecular and histological methods including multicolour immunohistochemistry (mcIHC) and immunofluorescence (mcIF), automated image analysis, laser capture microdissection, multispectral flow cytometry, and single-cell sequencing. 

MCIC supports academic and industry projects both nationally and internationally, ranging from investigator-driven basic research, to correlative studies for clinical trials, to fee-for-service projects with industrial clients.


Histology – Tissue fixation, paraffin embedding, sectioning, mounting, TMA construction, automated mcIHC and mcIF staining (up 8-colour), and laser capture microdissection.

Imaging – Advanced imaging suite with cutting-edge equipment, including a Vectra 3.0 automated quantitative pathology imaging system, Pannoramic MIDI digital slide scanner, and Vectra Polaris.

Analysis – Advanced image analysis software, including InForm (Perkin Elmer), HALO (Indica Labs) and QuPath, to quantify morphological and cellular features within tissue samples.

To inquire about histology services or to request a quote please contact Katy Milne (

Please consult the MCIC service guide for additional information.

Flow Cytometry

High dimensional flow cytometry – Two Cytek Aurora spectral flow cytometers (up to 48-parameter analyses) and dedicated computer workstations for multispectral data analysis.

Fluorescence activated cell sorting (FACS) – BD FACSMelody for single cell sorting

To inquire about flow cytometry services or to request a quote please contact Tyler Dyer (

Multicolour immunohistochemistry

Tertiary lymphoid structure in high-grade serous carcinoma.

(CD21: blue, CD3: green, CD20: red, CD208: black, PNAd: brown,CD8: darker purple)

High dimensional flow cytometry

tSNE projections of immune cell compartments from disaggregated HGSC cells colored by cell type and defined using common phenotypic markers.

Laumont CM, Wouters MCA, Smazynski J, Gierc NS, Chavez EA, Chong LC, Thornton S, Milne K, Webb JR, Steidl C, Nelson BH. Single-cell Profiles and Prognostic Impact of Tumor-Infiltrating Lymphocytes Coexpressing CD39, CD103, and PD-1 in Ovarian Cancer. Clin Cancer Res. 2021 Jul 15;27(14):4089-4100. PMID: 33963000.

Multicolour immunofluorescence

Tumour infiltrating lymphocytes in high-grade serous carcinoma.

(CD155: orange, CD68: magenta, PD-L1: cyan, CD3: yellow, CD8: green, PD-1: red, PanCK+: purple')
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