2 positions available May 2020 – Dec 2020 (8 months)
Co-op position 1 - Our lab has developed in house a synthetic model of T cell acute lymphoblastic leukemia by lentiviral transduction of hematopoietic stem progenitor cells from human umbilical cord blood. One co-op student will be working on investigating how various conditions contribute to this leukemic transformation. In this position, you will learn aseptic technique for culturing human primary cells, how to produce lentivirus and transduce cells, flow cytometry techniques and analysis, mouse necropsies and cell cryopreservation. We would prefer someone with wet lab experience and is organized and detail-oriented.
Co-op position 2 - Investigating the tumour ecosystem in human non-Hodgkin’s lymphoma. Using state-of-the-art single cell analysis (mass cytometry (CyTOF), single cell RNA sequencing and high-parameter flow cytometry), this project will investigate the heterogeneity of tumour cells and their surrounding non-malignant immune and stromal cells. This work is of direct clinical relevance in providing detailed phenotypic characterizations that are required in order to define biomarkers for lymphoma classification and prognosis. In this position, you will have opportunities to learn: 1) handling and processing clinical samples, including tissue digestion and culture of human primary cells; 2) maintaining and operating the CyTOF and multi-parameter flow cytometry; 3) Multi-dimensional data analysis. We prefer applicants who are detail-oriented and have a strong interest in bioinformatics/programming as such skills will allow for more involvement in data analysis.
Applicants may apply via their school's co-op program.