Application Deadline

Job Summary

The Aparicio lab is developing new single cell genome and transcriptome methods for tracking clonal trajectories at single cell level in patient tissues and to gain a better understanding of tumor microenvironment. Researchers uses the latest Single Cell Genomics technologies to rapidly identify genes that are involved in the development of cancer. This position will be working on the Single Cell Genomic research projects currently on-going in the research lab, providing technical guidance, expertise, and support to other internal/external researchers on Single Cell Genomics, in addition, helping with continual development and on-going production at 2 separate research facilities located in Vancouver, B.C. 

We are seeking a highly-motivated and highly skilled individual with expertise or experience in single cell genomics, robotics, next-generation sequencing and a strong background in molecular biology. The successful applicant will work full-time under the direction and supervision of both Dr. Samuel Aparicio, Principal Investigator, and the Laboratory Research and Operations manager. Individual should possess a strong molecular biology background; experience with liquid handling robotics is considered an asset. Experience with bioinformatics approaches to biological data and familiarity with handling of genomics and next-generation sequencing data will be an advantage. This position will provide the candidate with a high level of exposure to single cell genomics and translational research, as well as, providing opportunities to work collaboratively with industry partners and other researchers globally; position start date is flexible.


Organizational Status
Aparicio Lab is located in the department of Molecular Oncology at BC Cancer Research Institute in Vancouver, British Columbia, Canada. The candidate will report directly to Dr. Samuel Aparicio, or Laboratory Research and Operations Manager, in addition, the applicant may assist in overseeing technical work of students and trainees. More information on the lab, please visit https://aparicio.molonc.ca



Work Performed

Consequence of Error/Judgement
The work is of a complex nature and required high level of training, tasks are assigned and the incumbent exercises a considerable amount of responsibility, initiative and judgment in determining own work schedule to achieve those tasks. Errors may result in the permanent loss of irreplaceable tissue samples, loss of thousands of dollars of material and/or damage to equipment.  Incorrect decisions or actions may damage the reputation of the laboratory, lead to loss of credibility in this field, and be financially costly. Noncompliance with biosafety and chemical safety regulations may also result in closure of facility or loss of grant funding.

Supervision Received
The successful applicant will work under minimum supervision. From time to time, Principal Investigator or Laboratory Research and Operations Manager may provide specific instructions on unusual problems, or new project outlines. This position will report to the Principal Investigator or Laboratory Research and Operations Manager.   

Supervision Given
Depending on experience and expertise, the successful applicant maybe be required to assist in training and supervision of graduate students/trainees, junior staff, or external collaborators; including providing instruction on use of lab equipment and procedures, and will help troubleshoot experiments in their area of expertise.

Minimum Qualifications
Completion of a university degree in a relevant discipline or technical program and a minimum four years of related experience or an equivalent combination of education and experience. Some positions may require a graduate degree.

A MSc in Biological Sciences (Molecular Biology or other relevant field) or BSc with 4-5 years of related experience in academic research or biotech industry settings, preference will be given to applicants with work experience in single cell genomics biology or molecular biology.

Preferred Qualifications

  • Plans and performs experiments utilizing established complex procedures and/or techniques for investigating single cell genomics, involving the use of liquid handling robotics and 10x genomics platforms
  • Visualize and analyze cells using microscopy and flowcytometry.
  • Generation, preparation and submission of next-generation sequencing libraries.
  • Using a LMIS system to track and record sample data
  • Provide technical expertise and support in molecular biology, protein biochemistry, tissue culture and virology
  • Develop and implement standard operating protocols (SOPs).
  • Maintains laboratory records and experimental data.
  • Assist in general lab maintenance such as reagent preparation, receiving and inventory of lab supplies.
  • Conducts complex data analysis and interpretation of data resulting from high throughput experiments.
  • Presents data at scientific meetings, writes reports and contributes to writing ethics and grant applications.
  • Carries out any other related duties as assigned or required in keeping with the qualifications and requirements of this technician level.
  • A working knowledge and understanding of molecular biology and biochemistry techniques such as next-generation sequencing, PCR, DNA/RNA purification and cloning techniques is essential; prior working experience in next-generation sequencing and library construction is considered a great asset.
  • Experience with cell culture and aseptic technique.
  • Working hands-on experience in tissue handling and tissue dissociation methods would be an asset.
  • Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques including the following: RNA / DNA isolation, quantitative PCR, transfection, flow cytometry, immunohistochemistry, ELISA, protein isolation, and western blotting.
  • Preference will be given to applicants with liquid handling robotics, single cell genomics, next-generation sequencing, and 10x Genomics experience.
  • Experience in immunocytochemistry and basic data analysis in R or similar statistics package would be an asset.
  • Demonstrated understanding of experimental design and assay optimization.
  • Strong interpersonal skills, communication skills (orally and written) and the ability to interact positively and productively with other team members are essential.
  • Excellent documentation and organizational skills.
  • Able to multi-task, prioritize and handle multi-project assignments concurrently.
  • The successful candidate must be well-organized, conscientious, understand the importance of detail, and be able to multi-task and prioritize duties effectively.

Please include the following documents when applying:

  • Cover Letter
  • CV
  • Contact information for two or three references
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