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Martial Guillaud, PhD

— filed under:

Research Scientist - Imaging Unit, Integrative Oncology Adjunct Professor, Department of Statistics, Unversity of British Columbia

Rm 6-122 ▪ 675 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada

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  • Team

Jagoda Korbelic

Anita Carraro

Alan Harisson

Zhaoyang Chen

Monica Lee



Students

Fahime Sheikhzadeh, PhD Student, Biomedical Engineering Program, Department of Electrical and Computer Engineerin

Training
        • 1989 Cellular Biology (Animal GeneticS) Msc
        • 1990 Informatics and Statistics - Technology Degree 
        • 1993 Biomedical Engineering - PhD

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Teaching

        • Instruction in data analysis and in computers at the Joseph Fourier University (Biological Sciences) and Stendhal University (Social Sciences).
        • Instruction in Image analysis (statistics) for the French Institute National of Health and Medical Research (INSERM)

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A. Research Projects

We are interest in understand the relationship an date causality between molecular changes/perturbation and their phenotypic effects, at the nuclear, cellular, or tissular level.

     1- Oral cancer

In collaboration with Dr Catherine  Poh and Dr Miriam Rosin, we have been developing imaged-based technology to quantify changes occurring in oral pre-neoplasic lesions. 

     2- Cervical  Cancer

As part of two consecutive Po1 NIH grants, we have been developing, validating and testing optical and imaging technologies for the screening, detection and management of cervical pre-neoplastic lesions. Theses international multi-centers clinical trials are being conducting at the Vancouver General Hospital ,Vancouver, at the  MD Anderson Cancer Research Centre, and in the last year at the El Paso in Texas under the supervision of Dr Michele Follen and Dr Calum MAcAulay.

     3- Prostate Cancer 

     4- Breast  Cancer

We have initiating a collaboration with Dr Celia Marginean and Dr Soufiane El Hallani from the University of Ottawa, Department of Pathology and Laboratory Medicine to investigate the potential of image cytometry and QTP as a new diagnostic test in Barrett's Eosophagus lesions

    5- Barrett's esophagus

    5. Skin Epithelium

As part of a collaborative project with Dr Georgios Stamatas from Johnson&Johnson,Paris, we are investigating the topological and geometrical changes occurring in the early years in the infant skin epithelium using non invasive reflectance microscopy.

 

 

 

 

B. Technology

We have been developing a suite of algorithm for the imaging, processing and statistical classification of cytological and histological specimens. 

This suite is available for research only under recherché agreement for any academic use.

B.1 DUnit

B.2 Jclass

B.3 Asterics

B.4 Idefics Modeling Platform

  

 

 

Publications

Refereed Journals

2015
  •           Ngheim VT, Davies KR, Beck JR, Follen M, MacAulay C, Guillaud M, Cantor SB.  Economic evaluation of DNA ploidy analysis vs liquid-based cytology for cervical screening.  British Journal of Cancer.  2015 June 9;112(12):1951-7

     

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           El Hallani S, Guillaud M, Korbelik J, Marginean EC.  Evaluation of quantitative tissue pathology in the assessment of Barrett’s Esophagus associated dysplasia.  American Journal of Clinical Pathology.  2015 Jul; 144(1):151-64 [Epub ahead of print]


2014
  •        Li G, van Niekerk D, Miller D, Ehlen T, Garnis C, Follen M, Guillaud M, and MacAualy C.  Molecular Fixative Enables Expression Microarray Analysis of Microdissected Clinical Cervical Specimens Experimental and Molecular Pathology. Experimental and Molecular Pathology. 2014 Jan 15;5(2):485-9

  •        Arifler D, MacAulay  C,  Follen  M, Guillaud M. Numerical Investigation of two-light scattering patterns of cervical cell nuclei to map dysplastic changes at different epithelial depths. Biomedical Optics Express, Vol. 5 Issue 2, pp.485-498 (2014)

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               Baik J, Ye Q, Zhang L, Poh C, Rosin M, MacAulay C, Guillaud M. Automated Classification of oral premalignant lesions using image cytometry and random forests-based algorithms. Cell Oncol (Dordr). 2014 Jun;37(3):193-202

  •  

              Guillaud M, Buys TP, Carraro A, Korbelik J, Follen M, Scheurer M, Storthz KA, van Niekerk D, MacAulay CE.  Evaluation of HPV infection and smoking status impacts on cell proliferation in epithelial layers of cervical neoplasia.  PLoS One.  2014 Sep 11;9(9):e107088.

  •  

           Martinez V, MacAulay CE, Guillaud M, Lam WL, Corbett K, and Rosin PM, Targeting of chemoprevention to high-risk lesions: challenge and opportunity. Oral Oncology.  2014 Dec;50(12):1123-30               

2013
  • Contaldo M, Poh CF, Guillaud M, Lucchese A, Rullo R, Lam S, et al.  Oral Mucosa optical biopsy by a novel handheld fluorescent confocal microscope specifically developed: technologic improvements and future prostects.  Oral Surg Oral Med Oral Pathol Or Radiol.  2013 Dec;116(6):752-758
  • Arifler D, MacAulay  C,  Follen  M, Guillaud M Numerical analysis of dysplasia-associated  changes in depth-dependent light scattering profile of cervical epithelium. Proceedings of SPIE Clinical and Biomedical Spectroscopy and Imaging III, vol.8798 (2013)
  • Csikasz-Nagy A, Escudero LM, Guillaud M, Sedwards S, Baum B, Cavaliere M. Cooperation and competition in the dynamics of tissue architecture during homeostasis and tumorigenesis'. Seminarsin cancer biology 2013 Aug;23(4):293-8.
  • El Hallani S, Poh, C.,MacAulay C., Follen, M., Guillaud, M., Lane, P.  Ex vivo Confocal Imaging with Contrast Agents for the Detection of Oral Potentially Malignant Lesions. Oral oncology. 2013. 23415144

2012
  • Gibb EA, Becker-Santos DD, Enfield KS, Guillaud M, Niekerk Dv, Matisic JP, Macaulay CE, Lam WL.: Aberrant expression of long noncoding RNAs in cervical intraepithelial neoplasia. Int J Gynecol Cancer. 2012 Nov;22(9):1557-63
  • Khojasteh M, Buys TP, LeRiche J, Lam S, Guillaud M, MacAulay C.: A framework for quantitative assessment of Ki67 distribution in preneoplastic bronchial epithelial lesions. Anal Quant Cytol Histol. 2012 

Jun;34(3):120-38.

  •  Macaulay C, Poh CF, Guillaud M, Michele Williams P, Laronde DM, Zhang L, Rosin MP.: High throughput image cytometry for detection of suspicious lesions in the oral cavity. J Biomed Opt. 2012 Aug;17(8):086004-1.
  • MacLellan SA, Lawson J, Baik J, Guillaud M, Poh CFY, Garnis C. Differential expression of miRNAs in the serum of patients with high-risk oral lesions, Cancer Medicine. 2012;1(2):268–274.
  • Li, G., Guillaud, M., leRiche, J., McWilliams, A., Gazdar, A., Lam, S., and MacAulay, C.:  Automated sputum cytometry for detection of intraepithelial neoplasias in the lung.  Analytical Cellular Pathology35:187-201, 2012.
  • Buys, T.P.H, Cantor, S.B., Guillaud, M., Adler-Storthz, K., Cox, D.D., Okolo, C., Arulogon, O., Oladepo, O., Basen-Engquist, K., Shinn, E., Yamal, J.M., Beck, J.R., Scheurer, M.E., van Niekerk, D., Malpica, A., Matisic, J., Staerkel, G., Atkinson, E.N., Bidaut, L., Lane, P., Benedet, J.L., Miller, D., Ehlen, T., Price, R., Adewole, I.F., MacAulay, C., Follen, M.:  Optical Technologies and Molecular Imaging for Cervical Neoplasia: A Program Project Update, Gender Medicine, 9(1S):S9-S24, 2012. 

2011
  •  Yamal, J-M., Follen, M., Guillaud, M. and Cox, D.:  Classifying tissue samples from          measurements on cells with within-class tissue sample heterogeneity.  Biostatistics Advance          Access Oct; 12(4):695-709, 2011
  • Cantor, S., Yamal, J.M., Guillaud, M., Cox, D.D., Atkinson, E.N., Benedet, J.L., Miller, D., Ehlen, T., Matisic, J., vanNiekerk, D., Bertrand, M., Milbourne, A., Rhodes, H., Malpica, A., Staerkel, G., Nader-Eftekhari, S., Adler-Storthz, K., Scheurer, M.E., Basen-Engquist, K., Shinn, E., West, L.A., Vlastos, A-T., Tao, X., Beck, J.B., MacAulay, C. and Follen, M.:  Accuracy of optical spectroscopy fo rthe detection of cervical intraepithelial neoplasia: Testing a device as an adjunct to colposcopy. Int. J. of Cancer, Mar 1;128(5):1151-68, 2011.

2010

  • Guillaud, M., Clem, C., and MacAulay, C.:  Idefics: In silico Platform for the Study of Epithelial Preneoplastic Processes. Biosystems Oct;102(1):22-31, 2010.

  • Khojasteh, M., Guillaud, M., Buncher, R., and MacAulay, C.:  Spatial distribution of Ki67-expressing cells in the pre-neoplastic bronchial epithelial lesions.  Submitted to Cellular Oncology, 2010.
  • Lane, P.M., Guillaud, M., Lam, S., Standish, B., McWilliams, A., Vitkin, A., Yang, V., and MacAulay C.: Differential Shrinkage of Bronchial Epithelium due to Histological Processing, Submitted to Journal of Biomedical Optics, 2010.
  • Yamal, J-M., Follen, M., Guillaud, M. and Cox, D.:  Classifying tissue samples from measurements on cells: application in cervical neoplasia screening.  Submitted to Biostatistics 2010

2008

  • Shadeo, A., Chari, R., Lonergan, K., Pusic, A., Miller, D., Ehlen, T., Van Niekerk, D., Matisic, J., Richards-Kortum, R., Follen, M., Guillaud, M., Lam, W., and MacAulay, C.:  Up regulation in gene expression of chromatin remodelling factors in cervical intraepithelial neoplasia. BMC Genomics 9:64, 2008.
  • Guillaud, M., Zhang, L., Poh, C., Rosin, M., and MacAulay, C.:  Potential use of Quantitative Tissue Phenotype to Predict Malignant Risk for Oral Premalignant Lesions.  Cancer Research 68(9):3099-3107, 2008.

2007

  • Scheurer, M., Guillaud, M., Tortolero-Luna, G., MacAulay, C., Follen, M., Adler-Storthz, K.: Human Papillomavirus-related Cellular Changes Measured by Cytometric Analysis of DNA Ploidy and Chromatin Texture. Cytometry B Clin Cytometry, Sep;72(5):324-31, 2007.
  • MacKinnon, N., Lane, P., MacAulay, C., Guillaud, M., Stange, U.:  Hyperspectral imaging of H&E stained tissue sections using a spectrally programmable light engine. SPIE Symposium on Biomedical Optics (BiOS), Proc. of SPIE vol. 6441, pp. 64410A, Jan. 20-25, 2007.
  • Rosin, M., Poh, C., Guillaud, M., Williams, M., Zhang, L., MacAulay, C.:  Visualization and other emerging technologies as change makers for oral cancer prevention.  Annals of the New York Academy of Sciences.  Ann NY Acad Sci. 1098:167-83, 2007.
  • Collier, T., Malpica, A., Guillaud, M., Follen, M., Richards-Kortum, R.:  Real time reflectance confocal microscopy: Comparison of two dimensional images and three dimensional image stacks for detection of cervical precancer. J Biomed Optics 12(2):024021, 2007.
  • Van Niekerk, D., Guillaud, M., Matisic, J., Benedet, J.L., Rosbach K.J., Freeberg, A.J., Follen, M., MacAulay, C.:  p16 and MIB1 improves the sensitivity and specificity of the diagnosis of High Grade Squamous Intraepithelial Lesions:  methodological issues in a report of 447 biopsies with consensus diagnosis and HPV HCII testing.  Gynecologic Oncology 107(1);Suppl 1:S233-S240, 2007.
  • Thiberville, C., Guillaud, M., Lockwood, W., Lam, W., Follen, M., MacAulay, C.:  Multi-Scale System Biology Applied to Cervical Inter-epithelial Neoplasia. Gynecologic Oncology 107(1);Suppl 1:S72-S82, 2007.
  • Miller, D., Okolo, C., Mirabel, Y., Guillaud, M., Arulogun, A., Oladepo, O., Crain, B., Follen, M., Adewole, I.: Knowledge dissemination and evaluation in a cervical cancer screening implementation program in Nigeria. Gynecologic Oncology 107(1);Suppl 1;S196-S-207, 2007.
  • Scheurer, M., Guillaud, M., Tortolero-Luna, G., Follen, M., Adler-Storthz, K.:  Epidemiologic Modeling of Cervical Dysplasia with Molecular and Cytopathological Markers. Gynecologic Oncology, 107(1);Suppl 1:S163-S169, 2007.
  • Guillaud, M., Richards-Kortum, R., Follen, M.:  Paradigm shift: A new breed of pathologist. Gynecologic Oncology, 107(1);Suppl 1:S46-S49, 2007.

2006

  • Guillaud, M., Follen, M., MacAulay, C.:  Quantitative histopathological analysis of CIN sections.  Cellular Oncology 28(1/2):61-62, 2006.
  • Guillaud, M., Benedet, J.L., Cantor, S.B., Staerkel, S., Follen, M. and MacAulay, C.:  DNA ploidy compared to Human Papilloma Virus Testing (Hybrid Cature II) and Conventional Cervical cytology as a Primary Screening Test for Cervical High Grade Lesions and Cancer in 1555 Patients with Biopsy Confirmation.  Cancer 107(2):309-318, 2006.

2005

  • Swartz, RJ., West, LA., Boiko, IV., Malipica, A., Guillaud, M., MacAulay, C., Follen, M., Cox, D.,  Classification Using Cumulative Log Odds With an Example in Quantitive Pathology of Adenocarcinoma of the Cervix.  Gynecol. Oncol. 99 (Supp. 1):S24-S31, 2005.
  • Kamalov, R., Haskins, D., Guillaud, M., Harrison, A., Kemp, R., Chiu, D., Follen, M., MacAulay, C.:  A Java Application for Tissue Section Image Analysis.  Computer Methods in Programs in Biomedicine, 77(2):99-113, 2005.
  • Guillaud, M., leRiche, J., Dawe, C., Korbelik, J., Coldman, A., Wistuba, I., Park, I.W., Gazdar, A., Lam, S., MacAulay, C.:  Nuclear Morphometry as a Biomarker for Bronchial Intraepithelial Neoplasia:  Correlation with Genetic Damage and Cancer Development. Cytometry PartA 63A(1):34-40, 2005.
  • Scheurer ME, Tortolero-Luna G, Guillaud M, Follen M, Chen Z, Dillon LM, Adler-Storthz K. Correlation of human papillomavirus type 16 and human papillomavirus type 18 e7 messenger RNA levels with degree of cervical dysplasia. Cancer Epidemiol Biomarkers Prev. Aug;14(8):1948-52, 2005.
  • Follen, M., Crain, S., MacAulay, C., Basen-Engquist, K., Cantor, S.B., Cox, D., Atkinson, N., MacKinnon, N., Guillaud, M., Richards-Kortum, R.:  Optical Technologies for Cervical Neoplasia: Update of an NCI Program Project Grant. Clin. Adv Hematol Oncol. 3(1):41-53, 2005.
  • Guillaud, M., Adler-Storthz, K.,  Malpica, A., Staerkel, G., Matisic, J.,Van Niekirk, D., Cox, D.,  Poulin, N.,  Follen, M.,  and  MacAulay, C.:   Sub visual chromatin changes in cervical epithelium measured by texture image analysis and correlated with HPV.  4th International Conference on Cervical Cancer Gynecologic Oncology 99(3)(Supp. 1): S16-S23, 2005.
  • Malpica, A., Matisic, J., Van Niekirk, D.,  Crum, C.,  Staerkel, G., Yamal, G.M., Guillaud, M.,  Cox, D., Atkinson, E.N., Adler-Storthz, K., Poulin, N., MacAulay C., and Follen, M.:  Kappa statistics to measure interrater and intrarater agreement for 1790 cervical biopsy specimens amongst twelve pathologists: qualitative histopathologic analysis and methodologic issues.  Gynecologic Oncology 4th International Conference on Cervical Cancer  99(3)(Supp 1): S38-S52, 2005.

2004

  • Guillaud, M., Cox, D., Storthz, K.A., Malpica, A., Staerkel, G., Matisic, J., Van Niekerk D, Poulin, N., Follen, M., MacAulay, C.:  Exploratory analysis of quantitative histopathology of cervical intraepithelial neoplasia: objectivity, reproducibility, malignancy-associated changes, and human papillomavirus.  Cytometry PartA 60A(1):81-89, 2004.
  • Guillaud M, Cox D, Malpica A, Staerkel G, Matisic J, Van Niekerk D, Storthz KA, Poulin N, Follen M, MacAulay C.:Quantitative histopathological analysis of cervical intra-epithelial neoplasia sections: methodological issues. Cellular Oncology, 26(1-2):31-43, 2004.
  • Chiu, D., Guillaud, M., Cox, D., Follen, M., MacAulay, C.:  Quality Assurance Using Statistical Process Control:  an Implementation for Image Cytometry.  Cellular Oncology 26(3):101-117, 2004.
  • Yamal, J.M., Cox, D., Hittelman, W., Boiko, I., Malpica, A., Guillaud, M., MacAulay, C., Follen, M., Vlastos, A.T.:  Quantitative Histopathology and Chromosome 9 Polysomy in a Clinical Trial of 4-HPR.  Gyn Onc 92(2):296-306, 2004.

2003

  • Drezek, R., Guillaud, M., Collier, T., Boiko, I., Malpica, A., MacAulay, C., Follen, M., Richards-Kortum, R.:  Light Scattering from Cervical Cells Throughout Neoplastic Progression -Influence of Nuclear Morphology, DNA Content , & Chromatin Texture. Journal of Biomedical Optics 8(1):7-16, 2003.
  • Guillaud, M., Cox, D., Adler-Storz, K., Malpica, A., Staerkel, G., Matisic, J., VanNiekerk, D., Poulin, N., Follen, M., and MacAulay, C.:  Quantitative Histopathology of Cervical Intra-Epithelial Neoplasia. Reprinted from 5th International Multidisciplinary Congress, Eurogin 2003, Paris, France, pages 291-295, April 13-16, 2003.
  • Poulin, N., Frost, A., Carraro, A., Mommers, E., Guillaud, M., van Diest, PJ., Grizzle, W., Beenken, S.: Risk Biomarker Assessment for Breast Cancer Progression:  Replication Prescision of Nuclear Morphometry. Anal Cell Pathol. 25(3):129-38, 2003.
  • Arifler, D., Guillaud, M., Carraro, A., Malpica, A., Follen, M., Richards-Kortum, R.,:  Light Scattering from Normal and Dysplastic Cervical Cells at Different Epithelial Depths:  Finite Difference Time Domain Modeling with a Perfectly Matched Layer Boundary Condition.  Journal of Biomedical Optics 8(3):484-494, 2003.
  • Swartz, R., West, L., Boiko, I., Malpica, A., MacAulay, C., Carraro, A., Guillaud, M., Cox, D., Follen, M.:  Use of nuclear morphometry characteristics to distinguish between normal and abnormal cervical glandular histologies. Analytical Cellular Pathology, 25(4):193-200, 2003.
  • Lam, S., Xu, X-C., Parker-Klein, H., leRiche, JC., MacAulay, C., Guillaud, M., Coldman, A., Gazdar, A., Lotan, R.:  Surrogate endpoint biomarker analysis in a retinol chemoprevention trial in current and former smokers with bronchial dysplasia.  Int J Oncol 23:1607-1613, 2003.

2002

  • Lam S,MacAulay C, leRiche JC, Dyachkova Y, Coldman A, Guillaud M, Hawk E, Christen MO, Gazdar AF.  A randomized phase IIb trial of anethole dithiolethione in smokers with bronchial dysplasia.  J Natl Cancer Inst 94(13):1001-1009, 2002.

2000

  • Guillaud, M., MacAulay, C., leRiche, J., Dawe, C., Korbelik, J., Lam, S.:  In Optical Diagnostics of Living Cells III, 74 Proceedings of SPIE, Vol. 3921, 2000.

1999

  • A. Barranco-Mendoza, C. Clem, A. Gupta, P. Fizzano, and M. Guillaud, "Predicting the development of pre-invasive lesions from biopsies," Archives of Control Sciences, special issue on Control and Estimation in Biological and Medicine Sciences 9:25-40, 1999.

1998

  • A. Barranco-Mendoza, A. Gupta, C. Clem, M. Guillaud, P. Fizzano, R. Aggarwala, and F. Beardwood, "Predicting the development of pre-invasive neoplastic bronchial epithelial lesions from biopsies," Proceedings of the 5th International Conference of Mathematical Populations Dynamics. Gliwice, Poland, (1998). (Paper and invited talk)
  •   MacAulay C, Lam S, Klein-Parker H, Gazdar A, Guillaud M, Payne P, le Riche J, Dawe C, Band P, Palcic B:  Intermediate endpoint biomarkers for lung cancer chemoprevention.  SPIE 3260-28:207-211, 1998.

1997

  • Guillaud M, Matthews JB, Harrison A, MacAulay C, Skov K.  A novel image cytometric method for quantitation of immunohistochemical staining of cytoplasmic antigens.  Analyt. Cell. Pathol. 14:87-99, 1997.

1995

  • Guillaud M, Doudkine A, Garner D, MacAulay C, Palcic B.  Malignancy associated changes in cervical smears:  Systematic changes of cytometric features with the grade of dysplasia.  Anal. Cell. Pathol. 9:191-204, 1995.
  • Guillaud M, Marcelpoil R, Payne P, MacAulay C, Lam S, Palcic B.  Multi-scale cellular sociological analysis:  Application to the bronchial epithelium.  Proc. of Int'l. Workshop on Information Processing in Cells and Tissues, Liverpool, England, Sept. 6-8, p. 557-568, 1995.
  • Poulin N, Susnik B, Guillaud M, Doudkine A, Worth A, Palcic B.  Histometric texture analysis of DNA in thin sections from breast biopsies:  Application to the detection of malignancy associated changes in carcinoma in situ of the breast.  Analyt. Quant. Cytol. Histol. 17:291-299, 1995.

1994

  • Leger I, Guillaud M, Krief B, Brugal G.  Interactive computer assisted analysis of chromosome 1 colocalization with nucleoli.  Cytometry 16:313-323, 1994.

1993

  • Guillaud M, Marcelpoil R, Baujard O, Beaurepaire E.  Modele de simulation de l'emergence et de la croissance de tumeurs cancereuses dans un tissu sain differencie.  Premières Journées Francophones d'Intelligence Artificielle Distribuée et Systèmes Multi-agents, Toulouse, France, 7-8 Avril, 1993.

1992

  • Guillaud M, Chassery JM.  Histograms analysis by use of L-moments, linear functions of order statistics.  Statistique et Analyse des données 16:85-106, 1992.

1991

  • Guillaud M, Chassery JM.  Utilisation des L-moments pour l’approximation d’histogrammes de ploïdie issus d’un système d’analyse d’images.  XXIII ièmes journées de Statistique ASU, Strasbourg, 27- 30 Mai, 1991.