Haishan Zeng, PhD

hzeng@bccrc.ca     Rm 6-123 ▪ 675 West 10^th Avenue, Vancouver, B.C., V5Z 1L3, Canada

Current Appointments

• Distinguished Scientist, Imaging Unit – Integrative Oncology, BC Cancer Agency
• Associate Professor,  Dermatology and Skin Science,  UBC
• Associate Member,  Physics and Astronomy,  UBC
• Associate Member,  Pathology & Laboratory Medicine,  UBC

Training:

BSc - Beijing University/Peking University, Beijing China - Electronic Physics, 1979-1983

MSc - Institute of Electronics and Grad. School, University of Science & Technology of China, Chinese Academy of Sciences, Beijing, China - Electronic Physics and Devices, 1983-1986

PhD - University of British Columbia - Biophysics, 1989-1993

Lab Members:

Michael Short – Research Associate

Yasser Fawzy – Research Associate

Jianhua Zhao - Research Associate

Anthony Lee - Postdoctoral Fellow

Hequn Wang – Graduate Student

Kam Chow - Graduate Student

Hanna Pawluk - Graduate Student

Yunxian Tian - Graduate Student

Ali Majdzadeh - Undergraduate Student

Yingqiu Yu – Research Assistant

Wei Zhang – Research Assistant

Lioudmila Tchvialeva – Research Assistant

 Research Interests

My research interests are centered on the optical properties of biological tissues and light-tissue interaction as well as their applications in medical diagnosis and therapy. Biological tissues are optically inhomogeneous and multi-layered turbid media. When light interacts with tissue, it is scattered and absorbed, and the tissue may produce fluorescence. These interactions and the optical properties of tissue determine the distribution of incoming light inside the tissue, which is important for generation of any biological effects and for therapeutic applications. On the other hand, the optical properties and the effects of tissue on light are determined by the chemical composition, morphological structure, and physiological state of the tissue. Therefore, pathological changes in tissue affect the re-emitted optical signal detected when shining a beam of light on the tissue. Through many years of research, we have demonstrated that tissue pathology can be detected in vivo by optical methods non-invasively or minimum invasively through endoscopy. Several clinically relevant devices have been successfully developed for early cancer detection in skin, lung, and gastrointestinal tract.

We are currently exploring a couple of different types of optical measurement modalities for diagnostic applications:

• Diffuse Reflectance Spectroscopy and Multi-spectral Imaging, which explores elastic light scattering and light absorption by tissue chromophores such as melanin, hemoglobin, bilirubin, and water.
• Fluorescence Spectroscopy and Imaging, which explore molecular electronic energy transition-related light emission of certain biomolecules in tissue such as tyrosine, tryptophan, NADH, collagen, and elastin.
• Raman Spectroscopy, which explores inelastic light scattering to give fingerprint-like spectral signatures of molecular vibrations. Molecules in tissue that have unique Raman signatures include proteins, DNA, lipids, glucose, and melanin.
• In-Vivo Confocal Microscopy, which provides sectional images of tissue at micron spatial resolution, allowing us to visualize cellular structures and micro-circulation in real time on living tissue. Imaging mechanisms we are exploring include elastic scattering (reflectance), two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG).
• Laser Speckle Imaging, which analyzes interference patterns from light reflected off skin surfaces.

These researches may also lead to non-invasive assessment of treatment progress. We are applying optical methods to study the pharmacokinetics of photodynamic therapy (PDT) as well as to improve dosimetry for various phototherapy modalities including PDT.

In addition, we are applying MEMS (micro-electro-mechanical systems) technologies to miniaturize our optical devices for endoscopy uses and for point-of-care diagnostic applications. We are also applying nanotechnologies to enhance various light-tissue interactions for diagnostic and therapeutic applications, for example, using metal nanoparticle based SERS (surface enhanced Raman scattering) spectroscopy for diagnostics and developing nanoparticle based photo-thermal therapy.

Teaching:
UBC Phys 543 - Biomedical Optics http://www.phas.ubc.ca/~hzeng/index.html
SPIE SC 823 - Diagnostic Endoscopy http://spie.org/x1145.xml?course_id=M0000892

Software:
Vancouver Raman Algorithm
http://www.flintbox.com/technology.asp?page=3553

Publications:

2012

• Chen, Y., Zheng, X., Chen, G., He, C., Zhu, W., Feng, S., Chen, G., Li, Y., Huang, Z., Zeng, H., Chen, R.: Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering.  International Journal of Nanomedicine, 7:73-82, 2012
• Lin, J., Yu, Y., Li, B., Huang, H., Lin, S.,  Li, C., Su, Y., Feng, S., Chen, G., Li, Y., Huang, Z., Zeng, H., Chen, R.: Electrical pulse mediated enahnced delivery of sliver nanoparticles into living suspension cells for surface enhanced Raman spectroscopy.  Laser Physics Letters, 9:240-246, 2012.
• Lin, J., Chen, R., Feng, S., Pan, J., Li, B., Chen, G., Lin, S., Li, C., Sun, L., Huang, Z., Zeng, H.: Surface-enhanced Raman scattering Spectroscopy for Potential non-invasive nasopharyngeal Cancer Detection.  Journal of Raman Spectroscopy (published online October 27) 2011. (DOI: 10.1002/jrs.2072.)

2011

• Yu, Y., Lin, J, Wu, Y, Feng, S, Li, Y, Huang, Z, Chen, R, Zeng, H.: Optimizing electroporation assisted silver nanoparticle delivery into living C666 cells for surface-enhanced raman spectroscopy. Spectroscopy - An Internatioanl Journal 25:13-21, 2011
• Lin, D, Lin, J, Wu, Y, Feng, S, Li, Y, Yu, Y, Xi, G, Zeng, H, Chen, R.: Investigation of the interactions of lymphoma cells with paclitaxel by raman spectroscopy. Spectroscopy - An International Journal 25:23-32, 2011
• Wang, H, Huang, N, Zhao, J, Lui, H, Korbelik, M, Zeng, H.: Depth-resolved in vivo Micro-Raman Spectroscopy of a murine skin tumor model reveals cancer specific spectral biomarkers. Journal of Raman Spectroscopy 42:160-166, 2011.
• Feng, S, Chen, R, Lin, J, Pan, J, Wu, Y, Li, Y, Chen, J, Zeng, H.: Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser light.  Biosensors and Bioelectronics 26:3167-3174, 2011.
• Mansoor, H, Zeng, H, Chiao, M.: A micro fabricated optical scanner for rapid non-contact thickness measurement of transparent films. Sensors & Actuators: A: Physical, 167:91-96, 2011
• Lin, J, Chen, R, Feng, S, Pan, J, Li, Y, chen, G, Cheng, M, Huang, Z, Yu, Y, Zeng, H.: A novel blood plasma analysis technique combining membrane electrophoresis with silver nanoparticle based SERS spectroscopy for potential applications in non-invasive cancer detection.  Nanomedicine:Nanotechnology, Biology, and Medicine, 2011 (In press, doe:10.1016/j.nano.2011.01.012).
• Chen, J, Zhuo, S, Jian, X, Zhu, X, Zheng, L, Xie, S, Lin, B, Zeng, H.: Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease. Journal of Biomedical Optics, 16(5):051305 (1-6), 2011. (This paper has been selected for the May 15, 2011 Issue of Virtual Journal of Biological Physics Research, covering a focused area of frontier research)
• Short M, Lam, S, McWilliams, A, Ionescu, DN, Zeng, H.: Using laser raman spectroscopy to reduce false positives of autofluorescence bronchoscopies: A pilot study. Journal of Thoracic Oncology, 6:1206-1214, 2011.
• Mansoor, H, Zeng, H, Chiao, M.: Real-time thickness measurement of biological tissues using a microfabricated magnetically-driven lens actuator. Biomedical Microdevices, 13:641-649, 2011
• Ye Y, Chen, Y, Li Y, Su Y, Zou C, Chen Y, Ou, L, Chen, R, Zeng, H.: Characterization and discriminatino of nasopharyngeal carcinoma and nasopharyngeal normal cell lines using confocal raman microspectroscopy. Spectroscopy - An International Journal 25:214-224, 2011.
• Lin, D, Feng, S, Pan, J, Chen, Y, Lin, J, Chen, G, Xie, X, Zeng, H, Chen, R.: Colorectoal cancer detection by gold nanoparticle based surface-enhanced Raman spectroscopy of blood serum and statistical analysis, Optics Express 19(14):13565-13577, 2011.
• Lee, AMD, Wang, H, Yu, Y, Tang, S, Zhao, J, Lui, H, McLean, DI, Zeng, H.: In vivo video rate multiphoton microscopy imaging of human skin, Optics Letters, 36(15):2865-2867, 2011.
• Guze, K., Short, M., Zeng, H., Lerman, M., Sonis, S.: Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity. Journal of Raman Spectroscopy 42:1232-1239, 2011.
• Huang, N., Short, M., Zhao, J., Wang, H., Lui, H., Korbelik, M., Zeng, H.:  Full range charaterization of the Raman spectra of organs in a murine model. Optics Express 19:22892-22909, 2011. (This paper has been selected for publication int he Virtual Journal for Biomedical Optics, Vol. 7, Issue 1, Jan. 4, 2012)
• Huang, Z., Chen, X., Chen, Y., Chen, J., Dou, M., Feng, S., Zeng, H., Chen, R.: Raman spectroscopic characterization and differentiation of seminal plasma.  Journal of Biomedical Optics, 16:110501(1-3), 2011.
• Wang, S., Zhao, J., Lui, H., He, Q., Zeng, H.:  Monte Carlo simulation of near infrared autofluorescence measurements of in vivo skin.  Journal of Photochemistry and Photobiology B: Biology, 105:183-189, 2011.
• Mansoor, H., Zeng, H., Chen, K., Yu, Y., Zhao, J., Chiao, M.: Vertical optical sectioning using a magnetically driven confocal microscanner aimed for in vivo clinical imaging.  Optics Express, 19:25161-25172, 2011. (This paper has been selected for publication in the Virtual Journal for Biomedical Optics, Vol. 7, Issue 2, Feb. 1, 2012)

2010 

• Tchvialeva, L, Markhvida, I, Zeng, H, Lui, H, Lee, TK: Surface roughness measurement by speckle contrast under the illumination of light with arbitrary spectral profile Optics and Lasers in Engineering, Optics and Lasers in Engineering, 48:774-778, 2010.
• Feng, S, Chen, R, Lin, J, Pan, J, Chen, G, Li, Y, Chen, M, Huang, Z, Chen, J, Zeng, H: Nasopharyngeal Cancer Detection Based on Blood Plasma Surface-Enhanced Raman Spectroscopy and Multivariate Analysis, Biosensors and Bioelectronics, 25:2414-2419, 2010.
• Huang, N, Wang, H, Zhao, J, Lui, H, Korbelik, M, Zeng, H: Single-wall Carbon Nanotubes Assisted Photothermal Cancer Therapy – Animal Study with a Murine Model of Squamous Cell Carcinoma, Lasers in Surgery and Medicine, 42:638–648, 2010.
• Wang, S, Zhao, J, Lui, H, He, Q, Zeng, H: A Modular Raman Microspectroscopy System for Biological Tissue Analysis, Spectroscopy - An International Journal, 24:577-583, 2010 (This paper was selected as the cover article)

• Chen, G., Chen, J., Zhuo, S., Xiong, S., Zeng, H., Jiang, X., Chen, R., Xie, S.: Nonlinear spectral imaging of human hypertrophic scar based on two-photon excited fluorescence and second-harmonic generation, British J. of Dermatology, 161(1): 48-55, 2009.
• Cluff, GM, Short, MA, Lui, H, McLean, DI, Zeng, H, Korbelik, M, Chen, MX: Comparison of Connective Tissue Invaded by Lewis Lung Carcinoma to Healthy Connective Tissue by Means of Micro-Raman Spectroscopy, J. of Raman Spectroscopy, 40(8): 1087-1090, 2009.
• Guze, K, Short, M, Sonis, S, Karimbux, N, Chan, J, Zeng, H: Parameters Defining The Potential Applicability Of Raman Spectroscopy As A Diagnostic Tool For Oral Disease, J. of Biomedical Optics, 14 (1): 014016(1-9), 2009.
• Han, X., Lui, H., McLean, DI, Zeng, H.: Near-infrared autofluorescence imaging of cutaneous melanins and human skin in vivo, J. of Biomedical Optics, 14(2): 0240179(1-5), 2009.
• Liao, XH, Wang, Y, Chen, R, Gu, Y, Wang, YY, Zeng, H: Monte Carlo simulation of detecting the severity of port wine stains by spectroscopy technology, Acta Photonica Sinica, 38(5): 1254-1258, 2009.
• Lin, J, Chen, R, Feng, S, Li, Y, Huang Z, Xie, S, Yu, Y, Cheng, M, Zeng, H.: Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy, Biosensors and Bioelectronics, 25(2): 388-394, 2009 (doi:10.1016/j.bios.2009.07.027).
• Feng, S, Lin, J, Cheng, M, Li, YZ, Chen, G, Huang, Z, Yu, Y, Chen, R, Zeng, H: Gold Nanoparticle Based Surface Enhanced Raman Spectroscopy of Cancerous and Normal Nasopharyngeal Tissues under NIR Laser Excitation, Applied Spectroscopy, 63(10):1089-1094, 2009 (This paper was selected as the cover article).
• Chen, J, Lee, A, Zhao, J, Wang, H, Lui, H, McLean, DI, Zeng, H: Spectroscopic characterization and microscopic imaging of extracted and in situ cutaneous collagen and elastic tissue components under two-photon excitation, Skin Research and Technology, 15: 418-426, 2009 (DOI:10.1111/j.1600-0846.2009.00381.x).

• Chen, R., Huang, Z., Chen, G, Li, Y., Chen, X., Chen, J., Zeng, H.: Kinetics and subcellular localization of 5-ALA induced PpIX in DHL cells via two-photon excitation fluorescence microscopy, Intl. J. Oncology, 32:861-867, 2008.
• Short M, Lam S, McWilliams A, Zhao J, Lui H, Zeng H: Development and preliminary results of an endoscopic Raman probe for potential in-vivo diagnosis of lung cancers, Optics Letters, 33(7): 711-713, 2008.
• Zhao, J., Lui, H., McLean, D. I., Zeng, H.: Integrated Real-time Raman System for Clinical In Vivo Skin Analysis, Skin Research and Technology, 14: 484-492, 2008.
• Zeng, H, Zhao J, Short M, McLean D, Lam S, McWilliams A, and Lui H: Raman Spectroscopy for in vivo Tissue Analysis and Diagnosis – from Instrument Development to Clinical Applications. Journal of Innovative Optical Health Sciences, 1(1): 95-106, 2008.
• Tchvialeva, L, Lee, TK, Markhvida, I, Mclean, DI, Lui, H. and Zeng, H.: Using a zone model to incorporate the influence of geometry on polychromatic speckle contrast, Optical Engineering, 47(7): 074201(1-6), 2008.
• Fawzy, Y., Zeng, H.: Intrinsic fluorescence spectroscopy for endoscopic detection and localization of the endo-bronchial cancerous lesions, J. of Biomedical Optics, 13 (6): 064022(1-8), 2008.

• Markhvida, I, Tchvialeva, L, Lee, TK, and Zeng, H.: The Influence of Geometry on Polychromatic Speckle Contrast, Journal of Optical Society of America A, 24(1): 93-97, 2007.
• Chen, R., Huang, Z., Lui, H., Hamzavi, I., McLean, D.I., Xie, S., Zeng, H.: Monte Carlo simulation of cutaneous reflectance and fluorescence measurements —— the effect of melanin contents and localization, Journal of Photochemistry and Photobiology B: Biology, 86(3): 219-226, 2007.
• Huang, B., Chen, R., Zeng, H.: The Impact of Blood Content in Skin Tissue on Skin Spectra.   Spectroscopy and Spectral Analysis, 27(1):95-98, 2007.
• Siu, C.P.B., Zeng, H., Chiao, M.: Magnetically actuated MEMS microlens scanner for in vivo medical imaging, Optics Express, 15(18): 11154-11166, 2007.
• Zhao, J., Lui, H., McLean, D. I., Zeng, H.: Towards Instrument Independent Quantitative Measurement of Fluorescence Intensity in Fiber Optic Spectrometer Systems, Applied Optics, 46(29):7132-7140, 2007
• Zhao, J., Lui, H., McLean, D. I., Zeng, H.: Automated Autofluorescence Background Subtraction Algorithm for Biomedical Raman Spectroscopy, Applied Spectroscopy, 61(11):1225-1232, 2007

2006

• Hamzavi, I.,Shiff, N., Martinka, M., Huang, Z., McLean, DI., Zeng, H., Lui, H.: Spectroscopic Assessment of Dermal Melanin using Blue Vitiligo as an in vivo Model. Photodermatology, Photoimmunology & Photomedicine, 22:46-51, 2006.
• Fawzy, Y.S., Zeng, H.: Determination of scattering volume fraction and particle size-distribution in the superficial layer of a turbid medium using diffuse reflectance spectroscopy, Applied Optics, 45:3902-3912, 2006.
• Lane, P.M., Gilhuly, T., Whitehead, P., Zeng, H., Poh, C., Ng, S., Williams, M., Zhang, L., Rosin, M.P., MacAulay, C.E.: Simple Device for the Direct Visualization of Oral-Cavity Tissue Fluorescence. J of Biomedical Optics, 11: 024006(1-7), 2006.
• Short, M.A., Lui, H., McLean, D.I., Zeng, H., Alajlan, A., Chen, M.X.: Demonstrating Changes in Nuclei and Peritumoral Collagen within Nodular Basal Cell Carcinomas Via Confocal Micro-Raman Spectroscopy, J of Biomedical Optics, 11(3): 034004(1-9), 2006.
• Huang, Z, Zeng, H, Hamzavi, I, Alajlan, A, Tan, E, McLean, DI, Lui, H.: Cutaneous Melanin Exhibits Fluorescence Emission Under Near-Infrared Light Excitation. J. of Biomedical Optics, 11(3): 034010(1-6), 2006.
• Fawzy, Y.S., Petek, M., Tercelj, M., Zeng, H.: In-vivo assessment and evaluation of lung tissue morphologic and physiological changes from non-contact endoscopic reflectance spectroscopy for improving lung cancer detection, J. of Biomedical Optics, 11(4):044003(1-12), 2006.

2005

• Tercelj, M, Zeng, H., Mirjan, P., Rott, T., Palcic, B.: Acquisition of Fluorescence and Reflectance Spectra during Routine Bronchoscopy Examination Using the ClearVu EliteTM Device: Pilot Study. Lung Cancer, 50: 35-42, 2005.
• Huang, Z, Lui, H., McLean, DI, Korbelik, Zeng, H: Raman Spectroscopy in Combination with Background Near-Infrared Autofluorescence Enhances the In Vivo Assessment of Malignant Tissues. Photochemistry and Photobiology, 81: 1219-1226, 2005
• Lau, D.P., Huang, Z., Lui, H., Anderson, D.W, Berean, K., Morrison, M. D., Liang, S., Zeng, H.: Raman Spectroscopy for Optical Diagnosis in the Larynx – Preliminary Findings.  Lasers in Surgery and Medicine, 37: 192-200, 2005.
• Chen, R., Huang, B., Zeng, H.: The Optical Model of Human Skin.  Acta Laser Biology Sinica, 14(6):401-404, 2005.

2004

• Huang, Z., Zheng, W., Xie, S., Chen, R., Zeng, H., McLean, D.I., Lui, H.: Laser-induced autofluorescence microscopy of normal and tumor colonic tissue, Intl. J. of Oncology, 24: 59-64, 2004.
• Zeng, H., Petek, M., Zorman, M.T., McWilliams, A., Palcic, B., Lam, S.: Integrated Endoscopy System for Simultaneous Imaging and Spectroscopy for Early Lung Cancer Detection. Optics Letters, 29: 587-589, 2004.
• Zeng, H., McWilliams, A., Lam, S.:  Optical Spectroscopy and Imaging for Early Lung Cancer Detection: A Review. Photodiagnosis and Photodynamic Therapy, 1: 111-122, 2004.
• Huang, Z, Lui H, Chen MXK, McLean DI, Zeng, H.: Raman Spectroscopy of In Vivo Cutaneous Melanin. J. of Biomedical Optics, 9(6):1198-1205, 2004.
• Hamzavi, I.,Jain, H., McLean, D.I., Shapiro, J., Zeng, H., Lui, H.: Parametric Modelling of Narrow Band UV-B Phototherapy for Vitiligo Using a Novel Quantitative Tool, Arch. Dermatol. 140: 677-683, 2004.

2003

• Lau, D.P., Huang, Z., Lui, H., Man, C.S., Berean, K., Morrison, M.D., Zeng, H.:  Raman spectroscopy for optical diagnosis in normal and cancerous tissue of the nasopharynx - preliminary findings.  Lasers in Medicine and Surgery 32(3), 210-214, 2003.
• Korbelik, M., Sun, J., Zeng, H.:  Ischemia-reperfusion injury in photodynamic therapy-treated mouse tumors.  Br. J. Cancer 88, 760-766, 2003.
• Huang, Z., McWilliams, A., Lam, S., English, J. McLean, D.I., Lui, H. Zeng, H.  Effect of Formalin Fixation on the Near-Infrared Raman Spectroscopy of Human Bronchial Tissues, Intl. J. of Oncology, 23: 649-656, 2003.
• Huang, Z., McWilliams, A., Lui, H., McLean, D.I., Lam, S., Zeng, H.  Near-infrared Raman Spectroscopy for Optical Diagnosis of Lung Cancer, Intl J of Cancer, 107(6): 1047-1052, 2003.

2002

• Zeng, H., Korbelik, M., McLean, D.I., MacAulay, C., Lui, H.:  Monitoring photoproduct formation and photobleaching by fluorescence spectropscopy has the potential to improve PDT dosimetry with a verteporfin-like photosensitizer.  Photochemistry and Photobiology 75(4), 398-405, 2002.

2001

• Bissonnette, R., Zeng, H., Korbelik, M., McLean, D.I., Lui, H.:  Oral aminolevulinic acid induces protoporphyrin IX fluorescence in psoriatic.  Photochemistry and Photobiology 74(2);339-345, 2001.
• Huang, Z. Zeng, H., Hamzavi, I., McLean, D.I., and Lui, H.: A rapid near-infrared Raman spectroscopy system for real-time in vivo skin measurements.  Optics Letters 26(22):1782-1784, 2001.

2000

• Bissonnette, R., Shapiro, J., Zeng, H., McLean, D.I., and Lui, H.:  Topical photodynamic therapy with 5-aminolaevulinic acid does not induce hair regrowth in patients with extensive alopecia areata.  British Journal of Dermatology 143:1032-1035, 2000.

1999

• Zeng, H., Weiss, A., MacAulay, C., Cline, R.:  System for Fast Measurements of in vivo Fluorescence Spectra of the Gastrointestinal Tract at Multiple Wavelength Excitation. Appl. Optics 38(34):7157-7158, 1999.
• Morgan, D., Wilson, Janet E., MacAulay, C., MacKinnon, N., Kenyon, J., Gerlan, P., Dong, C., Zeng, H., Whitehead, P., Thompson, C., McManus, B.:  A new method for sensitive and reliable detection of heart allograft rejection:  Validation in the heteroptopic abdominal rat allograft model.  Circulation 100:1236-1241, 1999.

1998

• Bissonnette, R., Zeng, H., McLean, D.I., Schyeiber, W.E., MacAulay, C., Roscoe, D.L., Lui, H.:  Psoriatic plaques exhibit red autofluorescence that is due to protoporphyrin IX. Journal of Investigative Dermatology, 111:586-591, 1998.
• Zeng, H., Weiss, A., Cline, R., and MacAulay, C.:  Real time endoscopic fluorescence imaging for early cancer detection in the gastrointestinal tract.  Bioimaging 6:151-165, 1998.
• Zeng, H., MacAulay, C., McLean, D., Palcic, B., Lui, H.: The Dynamics of Laser Induced Autofluorescence Decay in Human Skin- Experimental Measurements and Theoretical Modeling. Photochemistry and Photobiology, 68(2):227-236, 1998.

1997

• Zeng, H., MacAulay, C., McLean, D.I. and Palcic, B.:  Reconstruction of in vivo skin autofluorescence spectrum from microscopic properties by Monte Carlo simulation.  J. Photochem. Photobiol. B: Biology 38:  234-240, 1997.

• Zeng, H., MacAulay, C., McLean, D.I. and Palcic, B.:  Spectroscopic and microscopic characteristics of human skin autofluorescence emission.  Photochem Photobiol. 61(6):639-645, 1995.

1993

• Zeng, H., MacAulay, C., Palcic, B. and McLean, D.I.:  A computerized autofluorescence and diffuse reflectance spectroanalyser system for in vivo skin studies.  Phys Med Biol 38(2):231-240, 1993.
• Zeng, H., MacAulay, C., McLean, D.I. and Palcic, B.:  Novel microspectrophotometer and its biomedical applications.  Optical Engineering 32(8):1809-1813, 1993.

• Zeng, H., Gong, Y.M. and Zhang, Z.:  Probe-hole field emission microscope system controlled by computer.  J. Electronics (Beijing, China, English version) 9(1), 1992.

• Gong YM, Zeng HS: Probe-hole field-emission microscope system controlled by computer. Chinese Physics Letters, 8(9): 446-449 1991.
• Gong, Y.M. and Zeng, H.:  Absorption and desorption of oxygen on the W(100) plane.  Surface Science 246:169-172, 1991.

• Zeng, H., Yang, L. and Ye, Y.:  Microcomputerized measurement and analysis of thermionic emission properties.  J. Electronics (Beijing, China), 10(2):1988; ibid. (English version) 6(2):1989.

• Yang, L., Zeng, H. and Ye, Y.:  A microcomputer controlled measurement system for measuring the thermionic emission properties.  Shanghai Vacuum Electronics (Shanghai, China), No. 3, 1988.

• Zeng, H. "Optical Properties and Autofluorescence Characteristics of Human Skin". In: Advances in Biomedical Photonics, Q. Luo (ed.), pp.73-99, HUST Press, Wuhan, China, May 2000.
• Zeng, H., “Microspectrophotometer, an Instrument for Microscopic Spectral Analysis”. In: Science, Technology and Education of Microscopy: an Overview, A. Méndez-Vilas (ed), pp.638-642, FORMATEX, Spain, March 2003.
• Zeng, H. and MacAulay C., "Fluorescence Spectroscopy and Imaging for Skin Cancer Detection and Evaluation". In: Handbook of Biomedical Fluorescence, M. Mycek and B.W. Pogue (eds.), pp.315-360, Marcel Dekker, New York, USA, April 2003.
• Tchvialeva, L., Zeng, H., Markhvida, I., McLean, D.I., Lui, H., Lee, T.K., “Skin Roughness Assessment”. In: Recent Advances in Biomedical Engineering, D. Campolo (ed.), pp.341-358, IN-TECH, Vienna, Austria, January 2010.
• Zhao, J., Lui, H., McLean, D.I., Zeng, H., “Real-Time Raman Spectroscopy for Noninvasive in vivo Skin Analysis and Diagnosis”. In: Recent Advances in Biomedical Engineering, D. Campolo (ed.), pp.455-474, IN-TECH, Vienna, Austria, January 2010.
• Zeng, H., “Characterization of Biomaterials”. In: Biomaterial for MEMS, M. Chiao and J.C. Chiao (eds.), Pan Stanford Publishing, Singapore, March, 2011.
• Chen, R., Lin, J., Feng, S., Huang, Z., Chen, G., Wang, J., Li, Y., Zeng, H.: Applications of SERS Spectroscopy for Blood Analysis. In: Applications of Raman Spectroscopy to Biology, Mahmoud Ghomi (ed.), pp. 72-105, IOS Press, Amsterdam, The Netherlands, March 2012.

• Zeng, H., Cline, R., MacAulay, C. and Jaggi, B.: Imaging system for detecting diseased tissue using native fluorescence in the gastrointestinal and respiratory tract. US Patent # 5,647,368, July 15, 1997.
• Zeng, H., Lui, H., MacAulay, C., Palcic, B. and McLean, D.I.: Spectrometer system for diagnosis of skin diseases.  United States Patent #6,008,889, December 28, 1999.
• Zeng H., Cline, R., MacAulay, C. and Jaggi B.:  Imaging system for detecting diseased tissue using native fluorescence in the gastrointestinal and respiratory tract.  Japanese Patent #3022377, February 28, 2000.
• Lui, H., Zeng, H., MacAulay, C., Palcic, B. and McLean, D.I.:  Fluorescence scope system for dermatologic diagnosis. United States Patent # 6,021,344, February 1, 2000.
• Zeng, H., Lui, H., MacAulay, C., Palcic, B. and McLean, D.I.:  Apparatus and methods relating to optical systems for diagnosis of skin diseases. United States Patent #6,069,689, May 30, 2000.
• Zeng, H., Lui, H., MacAulay, C., Palcic, B. and McLean, D.I.: Apparatus and method to monitor photodynamic therapy (PDT). United States Patent # 6,128,525, October 3, 2000.
• Whitehead, P., MacAulay, C., MacKinnon, N., Zeng, H., Thompson, C., McManus, B.:  Methods and apparatus for detecting the rejection of transplanted tissue.  United States Patent # 6,201,989, March 13, 2001.
• Lui, H., MacAulay, C., Zeng, H., McLean, D.I., Bisonnette, R.:  Photoactivation of endogenous porphyrins for treatment of psoriasis.  United States Patent # 6,269,818, August 7, 2001.
• Zeng, H.: Apparatus and methods relating to high speed Raman spectroscopy.  United States Patent #6,486,948, November 26, 2002.
• MacKinnon, N., Lam, S., Zeng, H., MacAulay, C.:  Apparatus for in vivo imaging of the respiratory tract and other internal organs.  United States Patent # 6,546,272, April 8, 2003.
• Zeng, H., Cline, R., MacAulay, C. and Jaggi, B.: Imaging system for detecting diseased tissue using native fluorescence in the gastrointestinal and respiratory tract. European Patent # EP0792618B1, October 8, 2003.
• Zeng, H., Lam, S., Palcic, B.:  Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices. United States Patent # 6,826,424, November 30, 2004.
• Zeng, H., Lam, S., Palcic, B.:  Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices. United States Patent # 6,898,458, May 24, 2005.
• Zeng, H., Lam, S., Palcic, B.:  Methods and apparatus for contemporaneous fluorescence and reflectance measurements with multiple measuring devices. Australian Patent # 2002215792, June 23, 2006.
• Zeng, H., Lam, S., Palcic, B.:  Imaging methods for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices. United States Patent # 7,115,841, October 3, 2006.
• Zeng, H., Lam, S., Palcic, B.:  Imaging methods for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices. United States Patent # 7,190,452, March 13, 2007.
• Zeng, H., Lam, S., Palcic, B.:  Image detection apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices. United States Patent # 7,253,894, August 7, 2007.
• Zeng, H.: In vivo Raman endoscopic probe and methods of use. United States Patent # 7,383,077, June 3, 2008
• Zeng, H., Lam, S., Palcic, B.:  Methods and apparatus for contemporaneous fluorescence and reflectance measurements with multiple measuring devices. Chinese Patent #ZL01822712.0, April 15, 2009.