Phenotype

The degree of abnormality of a cell can be defined by using image cytometry. Different features are computed for each nucleus :

• Shape features,
• Texture features,
• Densitometry features

Figure 6: Morphometric Index: Morphometric Analysis of 400 bronchial biopsies [View Image]

Linear combination of these features gives us a new "Morphometric index" (Figure 6), describing the degree of abnormality of the nuclei.
A Tissue Morphometric Index is also computed.

Genotype

Figure 8: Phenotype and Genotype [View Image]

• Loss of heterozygosity analysis of different tumor-suppressors genes ( 3p14.3, 3p21, 3p23-24, 3p25 9p, 5q, Rb, p53 ) are performed.
• This gives us information about the molecular state -function of the tissue, but no individual information about individual cells.