Dr. Christoph Bös, Diplom-Chemiker

I enjoy hiking, skiing, soccer and volleyball.

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Department:	Cancer Genetics (@ the BCCRC since 2000)
Research Role:	Staff Alumnus
Education:	Ph.D. (Microbiology), University of Tuebingen (Germany), 1997 Diplomchemiker (Biochemistry), University of Saarbruecken (Germany)
Birthplace:	Reimsbach (Saarland), Germany

Research Interests:

Structure-function relationship in Pgp and Spgp

My main interests are the multidrug resistant protein Pgp (MDR1) and the liver expressed bile acid transporter Spgp (BSEP). The structure-function relation of these proteins will be examined. The first step in these studies is the purification of both proteins. For Pgp several purification methods have been developed. The popular Baculovirus-insect cell-system in combination with the His-tag technique will be used to purify Pgp on a large scale. In co-operation with a crystallographic group we will try to solve the three dimensional structure of Ppg. The same purification method will be applied to Spgp. At the moment there is no existing purification method for Spgp. Therefore our knowledge about the structure and the function of Spgp is very limited. The purified protein can be used to characterize the transporter in respect to its substrates, its transport mechanism, and its kinetic and thermodynamic characteristics. One important characteristic of the ABC-transporter Pgp and Spgp is their assembly of two homologous halves. Studies by other research groups showed that these halves must interact which each other during substrate induced ATPase-transport-activity. Our Protein-Structure-Function Group is examining the arrangement and the interaction between these two halves by site directed mutagenesis and thiol-specific labeling of protein sites by fluorescence dyes. FRET studies should give information about interaction and conformational changes between the two halves and inside the nucleotide binding domains. Currently preliminary experiments about the function and localization of the natural cysteines of Pgp and Spgp are on the way.

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